Analysis of temperature generated by different irradiation protocols with violet LED and their effects on a 3D model-cultured odontoblast-like cells

• Grant number: 24/00773-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2024
• End date: June 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Felipe Sales de Oliveira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The main objectives of this study are: 1) to evaluate the whitening efficacy and temperature generated in enamel and pulp space through continuous or fractionated irradiation, performed for periods of 15, 30, and 45 minutes with violet LED; 2) to determine possible indirect damages caused by these violet LED irradiation strategies on MDPC-23 odontoblast-like cells in a 3D culture model. To achieve this, enamel/dentin discs with a thickness of 2.3 mm will be obtained, subjected to staining with black tea, and then adapted to artificial pulp chambers. For the negative control (NC), no irradiation will be performed, while in the positive control (PC), a commercial whitening gel with 35%H2O2 will be applied to the enamel. After completing the proposed therapies in this study, the color change of enamel/dentin discs will be evaluated (”E00 and ”WI; n=8), and the temperature generated on the enamel surface and inside the pulp chamber (thermocouple device, n=8) will be determined. To assess potential thermal damage to MDPC-23 odontoblast-like cells, a 3D framework will be formulated from a mixture of type 1 collagen, 10x culture medium, and sodium hydroxide (NaOH). The cells will be cultured in a 3D culture model, and after the therapies, they will be evaluated for viability (AlamarBlue, n=8); Live/Dead, n=4) and oxidative stress (EOx, n=8). The data will be subjected to specific statistical analysis.