Evaluation of bisphosphonate in bone modulation during the progression of periodontal disease. Study in experimental periodontitis in rats

• Grant number: 23/16329-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2024
• End date: March 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Rosemary Adriana Chierici Marcantonio
• Grantee: Gabriel Rosa Freire da Silva
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this project will be to evaluate the process of bone modulation in the progression of periodontal disease induced with or without the use of bisphosphonate. The animals will be randomly divided into 2 groups (7 animals in each group; n=7 ligatures/group): Control (C): The animals will be given serum daily. Bisphosphonate group (BIS): the animals will be given bisphosphonate (1mg/kg/day) daily. In both groups, the serum and the drug will be administered for 60 days. After this period, periodontal disease will be induced with ligatures on the upper right second molar. After 15 days of periodontal disease induction, the ligatures will be removed. The drugs will be administered until the time of euthanasia. At 7, 15 and 30 days, the animals will be euthanized. The specimens obtained from the first molar region will be decalcified, embedded in paraffin and stained with hematoxylin and eosin. Photographic images will be taken of the furcation and interproximal regions. Morphometric analysis will be carried out using a 30,000 ¼m2 grid with 50 points coinciding with the intersections of the horizontal and vertical lines. This grid will be positioned over the areas: A- Mesial and distal proximal regions of the second molar and B- Furcation. The cells present at each intersection will be defined. Next, a percentage analysis of each tissue component will be carried out in relation to the total number of points counted per experimental period, where the relative volumetric density (or proportion) of the following tissue components will be determined: bone, fibroblasts, inflammatory cells and blood vessels. The data generated by the stereometric analysis will be numerical. They will therefore be subjected to the Shapiro-Wilk Normality test to assess whether the data respects the distribution according to the central distribution theorem. If the data is normal, the parametric ANOVA test complemented by the Tukey test will be used for inferential analysis of the data. Otherwise, the non-parametric Kruskall-Wallis test supplemented by the Dunn test will be used to analyze the data (p< 0.05).