EFFECT OF THE APPLICATION OF MAGNETIC NANOPARTICLES AND HYPERTHERMIA IN OSTEOSARCOMA

• Grant number: 23/16012-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2024
• End date: July 31, 2026
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Sandra Yasuyo Fukada Alves
• Grantee: Vanessa Costa Gonçalves
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 19/08568-2 - Investigation of the extracellular vesicles (VEs) role in the initiation, propagation, regeneration, and modeling of biological mineralization, AP.TEM

Abstract

Osteosarcoma (OS) is a type of primary bone tumor that affects rapidly growing regions of the bone, which contributes to incidence and initial peak prevalence between 0 and 19 years of age. Treatment of OS consists of surgical resection and chemotherapy, which are not specific to the tumor cell. Therefore, new approaches are being studied in order to assist in the treatment, which includes the use of magnetic nanoparticles (NPM). This technique stands out due to high biocompatibility, therapeutic safety and efficacy. The present project aims to evaluate the effect of treating OS with magnetite NPM in vitro and in vivo experimental models. Initially, the biocompatibility of magnetite NPM in MC3T3 osteoblastic cells and OS lines (U2 and MG-63) will be evaluated. For this, the cells will be treated with different concentrations of magnetite NPM and cell viability will be determined. Next, the cells will be exposed to NPM concentration and subjected to the Theranostic Platform. The objective is to raise the temperature in a controlled manner and apply a magnetic field directed to the cells, with the aim of selectively evaluating the induction of cell death in tumor cells. This effect will be analyzed by cell viability, comparing osteoblastic cells and tumor cells. In a next step, magnetite NPM will be injected into the tumor followed by exposure to the Theranostic Platform. This approach will provide data on the location of injected NPM, morphological images and allow monitoring of tissue temperature. Furthermore, it will allow non-invasive assessment of the effectiveness of the treatment in eliminating tumor cells.