MACROPHAGE POLARIZATION AFTER APPLICATION OF EARLY AND DELAYED ORTHODONTIC FORCE IN TRAUMATIZED TEETH

• Grant number: 24/09591-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2024
• End date: August 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Orthodontics
• Principal Investigator: Guido Artemio Maranon Vasquez
• Grantee: Carol Silva Gaspar
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This research proposal aims to investigate the effect of applying orthodontic force on traumatized teeth of rats, after observation periods of 7 and 60 days, on the mechanisms involved in the process of dental repair / resorption, with emphasis on the behavior of macrophages. A model of tissue damage resulting from avulsion of upper incisor and treated with immediate replantation will be studied. Wistar rats will be randomly allocated into 4 groups (n = 24) according to the observation period after trauma and the application of orthodontic force. The animals will be euthanized after 1, 3, 7 and 14 days of force application. The following analyses will be performed: microtomographic analysis and morphometric analysis using optical microscopy to characterize resorption of the mineralized tissue; histopathological analysis with H&E; assessment of activity of clastic cells by TRAP histoenzymology; and identification of M1-like- (CD68+, iNOS+) and M2-like-macrophages (CD68+, CD163+), and associated markers (IFN-g, TNF-a, IL-1B, RANKL, IL-4, IL-10, Arg 1, OPG), by immunofluorescence and immunohistochemistry analyses, respectively. The data will be submitted to statistical analysis adopting a significance level of 5%.