Bioactivity of materials for pulp regeneration on cellular response and protein synthesis in stem cells from primary teeth

• Grant number: 23/16826-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2024
• End date: May 31, 2028
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Ana Beatriz Vieira da Silveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The purpose of this study will be to analyze the cellular response and protein synthesis by different types of stem cells from exfoliated human deciduous teeth for dentin-pulp regeneration with different pulp therapy materials and a new bioactive product (BBio). Samples will be obtained through primary culture of stem cells from exfoliated human primary teeth. The methodology will be divided into two experimental phases: In the first experimental phase, stem cells from the pulp of exfoliated human deciduous teeth (SHED) will be collected, cultivated and characterized. Such cells will be tested in contact with extracts of the following bioactive materials: BBio Membrane (G1), Biodentine (G2) and Cimmo HD® (G3). In the second experimental phase, stem cells from the dental papilla of exfoliated human deciduous teeth (SCAPd) will be collected, cultivated and characterized. Such cells will be tested in contact with extracts from the following filling pastes: Calcium Hydroxide® paste (G1), FeaPex® paste (G2) and Guedes-Pinto® paste (G3). Cell viability, cell migration, pH analysis and immunofluorescence experiments will be carried out. As well as gene expression and protein synthesis for odontogenic, osteogenic and angiogenic markers using the RT-PCR method and immunoenzymatic assay, respectively. In the statistical analysis, as these are quantitative measures, the Shapiro-Wilk normality test will be applied. For data with normal distribution, intragroup and intergroup comparison will be performed using the repeated measures analysis of variance test, followed by the Tukey test. For data that do not present a normal distribution, intragroup comparison will be performed using the Friedman test and intergroup comparison using the Kruskal-Wallis test. Pearson Correlation will be applied to measure the correlation between measurements. Multivariate mixed effects linear regression analysis will be applied to evaluate the results found. For all tests, the significance level adopted will be 5%.