Evaluation of early development and toxicological effects following direct and indirect exposure to nitrosodimethylamine in second-generation (F1) Wistar rats.

• Grant number: 24/03964-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2024
• End date: August 31, 2025
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Arielle Cristina Arena
• Grantee: Giovanna Ferrari Rosalem
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 22/15364-7 - Implications of parental exposure to nitrosodimethylamine in rats: immediate and multigenerational effects on toxicological and reproductive parameters, AP.R

Abstract

Currently, about 15% of the world's population faces infertility problems, and many of these issues can be attributed to environmental factors and lifestyle. In addition, evidence indicates that infertility can begin to develop already in intrauterine life, where there is greater susceptibility to the effects of environmental stressors, considering that from the preconception period to puberty there is a high metabolism and maturation processes in progress. According to DOHAD (Origins of the Development of Health and Disease) and POHAD (Paterly Origins of the Departure and Disease), which study hypotheses of exposures to parents and the relevance to the development of the offspring, exposure to potentially harmful substances at various stages of life can have immediate effects or manifest itself Only in adult life or in future generations. Among the environmental pollutants that can compromise maternal and fetal progress, the project focuses on N-Nitrosodimethylamine (NDMA), a compound of the group of M-nitrosamine, found in the environment, in food, drinking water and as a contaminant of medicines, making human exposure almost inevitable. Our goal is to investigate the consequences of exposure to Nitrosodimethylamine, via the paternal, maternal and combined organism (paternal and maternal), added to the postnatal exposure of the offspring to NDMA in toxicological, developmental and reproductive parameters of female rats of the F1 generation. For this, the parental generation (FO) will be exposed to the control (1 mL/ce of distilled water) or 7.2 ng/kgidia of NDMA (via orall), of the postnatal day (DPN) 60 to 90, during the mating period, gestational and lactational period. After birth, the offspring will e evaluated in relation to their initial sexual development and will be continuously exposed to control (1(sex/litter) and NDMA (1/sex/litter) until DPN 60. The female offspring will also be evaluated in relation to the installation of puberty, estral cycle, sexual behavior, fertility test and hematological parameters.