The potential for chondrogenic differentiation of mesenchymal stromal cells from the pulp of deciduous teeth with and without the application of low-power laser.

• Grant number: 24/10361-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2024
• End date: November 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Daniela Franco Bueno
• Grantee: Ygor Goncalves Felix de Mattos
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil

Abstract

The temporomandibular joint (TMJ) is formed by the chewing muscles and the condyle of the jaw, which connects to the temporal bone through the mandibular fossa. Anatomically, any disorder caused in this region can lead to pain, such as disordersmyofascials, osteoarthritis and disc disorders. Thus, several treatments are observed to solve the problem, being these invasive as surgical treatments, non-invasive treatments, such as anti-inflammatory drugs.In this regard, the search for new sources of treatment become necessary, such as the use of mesenchymal stem cells extracted from the pulp of deciduous teeth that, by presenting infinite osteogenic, chondrogenic, adipogenic, dentinogenic and neurogenic potential, become excellent cells for use in tissue engineering.In vitro chondrogenic differentiation of mesenchymal stem cells can be used in regenerative medicine and the application of low-power laser during this chondrogenic differentiation process can enhance this differentiation capacity. In addition, the association of laser use during chondrogenic differentiation is an investment that can bringbetter conditions for the regeneration of TMJ cartilaginous tissues if an association of the use of stem cells to the use of low-power laser to treat TMJ pathologies occurs.Thus, the present study aims to describe and compare the potential for chondrogenic differentiation of stem cells from pulp of deciduous teeth, associated or not with the application of low-power laser (red and infrared together) with different energies supplied to cells (0.3 J; 2J; and 4J), in order to evaluate whether the application of laser can increase the chondrogenic potential of cells so that new technologies for treatment of temporomandibular disorders (TMD) can be developed in the future.