Analyzing the role of HDL in activated macrophages: a proteomic approach

• Grant number: 24/04000-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: December 01, 2024
• End date: February 28, 2029
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Graziella Eliza Ronsein
• Grantee: Francielle Aguiar Gomes
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Macrophages are cells of the immune system with an important role in inflammatory processes and are generally studied using the THP-1 immortalized cell line as a model, however, how close these cells are to primary cells is unknown. High-density lipoprotein (HDL) has a relevant, but poorly understood, anti-inflammatory role, especially for these cells. Thus, the project proposes a comparison study of the THP-1 cell line model with monocytes isolated from peripheral blood, using the SILAC technique and, in addition, a thorough proteomic analysis of the influence of HDL in an inflammatory context on macrophages using spatial proteomics technique. Finally, we aim to study the different polarizations of macrophages through single-cell proteomics.