Role of the kinin B1 receptor in specific regions utilizing the animal's tissue-specific conditional knockout

• Grant number: 25/01531-7
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2025
• End date: June 30, 2029
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Ronaldo de Carvalho Araújo
• Grantee: Luisa Furtado Hummel
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 21/14313-7 - Role of kallikrein kinin system on metabolism and repercussion on voluntary exercise, AP.TEM

Abstract

The kallikrein system (KKS) has been studied in inflammatory processes, with special attention to kidney damage in addition to blood pressure control. However, the central role of kinin B1 and B2 receptors is little known. In order to spatially identify the expression of SCC components indifferent nuclei of the hypothalamus we will use fluorescent in situ hybridization (RNAscope) in male and female mice. This approach will allow the identification of SCC components indifferent nuclei of the hypothalamus. A floxed B1 animal is being generated in collaboration with Germany and we will use it to generate B1 animals deficient in specific tissue using the Cre-loxP technique. We thus aim to combine the RNAscope technique and genetically modified animals (Cre-LoxP system) to identify possible neuronal and/or glial phenotypes.