Bromatological, microbiological and shelf life parameters of frozen pangasius fillets sold in Presidente Prudente-SP

• Grant number: 24/20046-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2025
• End date: December 31, 2025
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Ricardo Firetti
• Grantee: Lorraine Pissinin Dutra
• Host Institution: APTA Regional. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

Considering that pangasius was the second main fish species imported by Brazil in 2023, behind only salmon, this proposal aims to investigate qualitative parameters of frozen fillets of this fish from Vietnam sold in retail in the city of Presidente Prudente/SP. Thus, variables related to bromatological composition; icing (freezing); presence/absence of pathogenic bacteria and lipid oxidation (quality deterioration) will be investigated in 05 brands available for sale in supermarkets. For this purpose, products will be purchased directly from physical retail stores in three replicates with a time interval of 60 days each, observing the criterion of 20 fillets (sampling units) belonging to the same batch for each commercial brand. Such products will be stored in freezers at the UNOESTE Animal Nutrition and Food Analysis Laboratory. In the icing tests of pangasius fillets, the international methodology provided for in the Codex Alimentarius standards will be used. Regarding the microbiological parameters, the methodology provided for in ANVISA RDC No. 724, of July 1, 2022, and ANVISA IN No. 161, of July 1, 2022, will be used. Finally, the shelf life tests will be performed following the antioxidant activity method in DPPH and the FRAP method to measure lipid oxidation. Additionally, the price per gross kilogram of product will be corrected using the net weight after deglazing to verify the value actually paid for the thawed fillet. The results obtained will be analyzed according to their respective methodologies; however, we will make a special tabulation in order to jointly evaluate a wider range of data through multivariate statistical techniques (factor analysis, correspondence, and block clustering). The names of the trademarks will not be disclosed. It should be added that this proposal derives from research funded by FAPESP through Process 2022/14453-6.