3D Bioprinting model in the study of autism: effects of valproic acid and possible protection with cannabidiol

• Grant number: 25/04214-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2025
• End date: January 31, 2029
• Field of knowledge: Biological Sciences - Pharmacology - Neuropsychopharmacology
• Principal Investigator: Vanessa Costhek Abílio
• Grantee: Camila Estevam Manzini
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Neurogenesis is essential for the formation of brain structures, and alterations in this process, caused by environmental factors such as exposure to valproic acid (VPA), are associated with autism. Although animal models are widely used, they have limitations in replicating the complexity of the human brain. In this context, 3D bioprinting emerges as a promising alternative, enabling the creation of three-dimensional neural tissues from human-induced pluripotent stem cells (hiPSCs), allowing the study of cellular organization and neuronal differentiation. Additionally, cannabidiol (CBD) stands out as a promising therapeutic approach due to its potential neuroprotective effects. Thus, this study aims to develop a 3D bioprinting model to investigate the effects of VPA on neurogenesis through analyses of cell viability, proliferation, and tissue organization, as well as to evaluate the possible modulatory effects of CBD. The project also considers biological differences between XX and XY individuals, aiming to better understand the cellular basis of autism and explore new therapeutic strategies.