CHARACTERIZATION OF ADHESIVES CONTAINING CANESTAT AND EVALUATION OF THEIR EFFICACY IN PROTECTING DENTIN INTERFACES FROM EROSION

• Grant number: 24/18482-6
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 01, 2026
• End date: June 30, 2026
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Vinícius Taioqui Pelá
• Supervisor: Marcella Esteves Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University Hospital Tübingen, Germany
• Associated to the scholarship: 22/13268-0 - Evaluation of the protective potential of CaneCPI-5, Stn15pSpS or Hb, alone or in combination against erosive challenges in vivo: comparison between mouthwash solutions and orodispersible films, BP.PD

Abstract

The use of organic components has gained significant momentum for the prevention of dental erosion. Recently, a novel hybrid protein (CaneStat) was recombinantly produced by our research group combining a protein derived from sugarcane with a peptide from statherin. However, there are no studies investigating the protective effect of this protein in safeguarding the interface between dental restorations and dentin from erosive challenges. Thus, the aim of this study will be to evaluate the efficacy of adhesives containing CaneStat in protecting against erosion at the interface between dentin and resin. The CaneStat protein will be produced using recombinant techniques (E. coli). This study will be conducted in 2 parts using experimental MDP-based (Methacryloyloxydecyl dihydrogen phosphate) self-etching dentin adhesives systems (DAS), as follows: 1) DAS without protein (negative control); 2) Clearfil SE Bond (commercial positive control); 3) DAS with 0.01 mg/mL CaneStat; 4) DAS with 0.1 mg/mL CaneStat; 5) DAS with 1 mg/mL CaneStat. PART 1 (In Brazil): To characterize the novel DAS above, the analyses of surface wettability (n=10/group) and degree of conversion (n=3/group) will be conducted. The micro-shear bond strength (µSBS) test will be used to evaluate the adhesion strength on sound and eroded dentin samples (n=12/group). PART 2 (BEPE - Switzerland): The following protocol will be developed at the dentin interface, testing the groups described above: Six pH cycles per day will be conducted for five consecutive days, involving immersion of the samples in citric acid (0.05 M, pH 2.3) for 5 min, 10 mL, 170 rpm. To simulate the remineralization process, the samples will be stored in artificial saliva, between cycles for 60 min, 170 rpm, at 22 °C. The samples will be rinsed with distilled water between the cycle for 5 s. The response variables for assessing erosion protection at the interface between dentin and resin will include (n=20/group) Table-Top Reflectometry, Optical Non-Contact 3D Profilometry, and Scanning Electron Microscopy. Data will be analyzed using appropriate statistical tests following evaluations of normality and homogeneity (p<0.05). (AU)