In vitro and in vivo characterization of 3D printed nerve guidence conduits for peripheral nerve regeneration

• Grant number: 25/04600-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2025
• End date: January 31, 2028
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Juliana Marchi
• Grantee: Larissa Ribeiro Lourenço
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• Associated research grant: 24/11219-8 - Development of nerve guide conduits based on chitosan and bioactive glass for application in peripheral nerve regeneration, AP.R

Abstract

Peripheral nerve injuries, if not properly treated, lead to loss of biofunctionality, mobility, and quality of life. The current gold standard for treatment is autologous grafting, but this approach has drawbacks such as donor site morbidity and low functional recovery rates. Tissue engineering-based scaffolds offer a promising alternative to overcome these limitations. This project focuses on developing and characterizing 3D-printed chitosan-based nerve guidence conduit (NGCs) incorporating bioactive glass (BG) to enhance peripheral nerve regeneration (PNR). Chitosan provides a biomimetic environment, while BG releases ions that may promote PNR through cellular signaling. Additionally, 3D printing enables the fabrication of complex, patient-specific structures. The project objectives include: (i) evaluating material sterilization; (ii) optimizing the 3D printing process; (iii) assessing NGC properties for nerve tissue compatibility; and (iv) conducting in vitro and in vivo studies to evaluate biocompatibility, regeneration rates, and biofunctionality. Ultimately, this research aims to develop optimized NGCs with superior properties, providing an effective alternative to currently available materials for PNR.