Analysis of clinical and blood biochemical parameters as a function of transcutaneous vascular photobiomodulation in extraction of lower third molars - Randomized Clinical Trial

• Grant number: 25/11653-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2026
• End date: February 28, 2027
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Leonardo Perez Faverani
• Grantee: Mileni Buzo Souza
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Third molar extraction is the most common surgical procedure performed by oral and maxillofacial surgeons, and the most common complications associated with it are pain, swelling, and trismus. In this sense, Modified Intravascular Laser Blood Irradiation (modified ILIB) has been the subject of study and appears to show promising results in controlling pain and inflammation. The objective is to evaluate the potential clinical and systemic effects of modified ILIB in individuals undergoing lower third molar extraction. For this purpose, 30 participants, aged between 16 and 35 years, without systemic comorbidities, with indication for extraction of elements 38 and 48, will be included in this split-mouth clinical trial. After selecting the participants, randomization will be performed, in which the sequence of the choice of the side to be operated on (tooth 38 or 48) and the group (ILIB or PLACEBO) will be determined. The extractions will be performed at three-week intervals in each individual. The volunteers and the surgeon/researchers, who will be involved in the analyses and interpretations, will not know which group the participant belongs to (double-blind). For the analyses, blood will be collected in the immediate preoperative period and 48 hours postoperatively, and the samples will be used to evaluate inflammatory biochemical parameters (prostaglandin E2, C-reactive protein, tumor necrosis factor alpha, and interleukins 1¿, 6, and 10), oxidative (superoxide dismutase enzyme) and vasoactive (nitric oxide) parameters, in addition to pain assessment using the Visual Analog Scale (6, 12, 24, 48, 72 hours, and 7 days after surgery), maximum mouth opening, edema (preoperative and 48 hours postoperative), and postoperative quality of life. The data obtained will be submitted to the Shapiro-Wilk test to choose a parametric or non-parametric test, considering a significance level of 5%. (AU)