Abstract
The pst operon (or pstSCAB-Phou) E. coli belong to the pho regulon, which in turn responds to external stimulus limitation phosphate (Pi). Pst the genes coding for a transport system Pi of high affinity, which also acts as a repressor of pho regulon capable of the abundance of Pi in the middle. In this design will be isolated pst spontaneous mutants in a culture medium containing glycerol…