Abstract
The aim of this study will be to investigate de enficiency of controled atmosphere (5% CO2, 5% O2 and 90% N2) on oocyte maturation with defined cuture medium or supplemented with porcine folicular fluid (PFF) and also on in vitro embryo development. Oocytes will be in vitro matured (IVM) for 44 hours in two culture medium: TCM199 supplemented with PVA 0,1%, or PFF10% with controlled atmo…