Abstract
Urea denatures proteins at different concentrations, depending on the experimental conditions and the protein. We investigated the pressure-induced denaturation of bovine serum albumin (BSA) in the presence of subdenaturing concentrations of urea based on a two-state equilibrium. Pressure-induced denaturation was enhanced at urea concentrations ([U]) of 3.5 M to 8.0 M, with the free energ…