Abstract
Aim: Develop a low-cost reaction mixture for Quantitative PCR (qPCR) with specificity, efficiency, and sensitivity equivalent to the foreign commercial kits. Methods: Mixtures will be with varied concentrations of SYBR® Green, MgCl2 and Platinum Taq DNA Polymerase Brazil (core reagents). The additives bovine seric albumin, dimethyl sulfoxide, formamide, polyethylene glycol 400, polyoxyeth…