Abstract
This project aims to develop a protocol for oocyte freezing Prochilodus lineatus. To this solution will be evaluated three extensor (Ringer's solution, Hank's solution, and phosphate buffer) and three cryoprotectant substances (Dimethyl sulfoxide, propylene glycol and methanol) at a concentration of 0.2M to 4M linked glucose. The oocytes are packaged in straws of 0.5 ml and exposed to a c…