Abstract
The aim of this proposal is to develop a multiplex-nested-RT-PCR capable of simultaneously detecting five RNA viruses associated with congenital infections: rubella virus, enterovirus (poliovirus, echovirus, coxckasie virus, other unclassified enterovirus) and three arboviruses (dengue, chikungunya and zika). Positive amplifications will be confirmed by Real Time PCR targeting only the pa…