Graduated in Biomedicine from the University of Mogi das Cruzes (UMC), she is currently pursuing a direct PhD in Biotechnology, studying cell death in yeast at the Interdisciplinary Center for Scientific Research (CIIB). Her focus is on the production of recombinant metacaspase in a heterologous system in E. coli and site-directed mutations, aiming to evaluate the interaction of calcium for the activation and control of enzymatic activity, using techniques such as Polymerase Chain Reaction (PCR), Western Blot, and Intrinsic Fluorescence. (Source: Lattes Curriculum)
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The present research project aims at the production of Saccharomyces cerevisiae (YCA1) metacaspase, including mutations in the site directed at the aspartate residues responsible for the interaction of alcohol with this enzyme. A YCA1 metacaspase undergoes automatic processing to become catalytically active, and uses calcium as an enzymatic co-factor both for its automatic processing and …
The cell cycle is determined primarily by the processes of division, differentiation and death, in organisms such as mammals it is described that cell death is linked to the action of enzymes known as caspases. In recent years enzymes of the caspase family have been described that are involved in programmed cell death in simple eukaryotic organisms such as fungi and protozoa (metacaspases…
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