Abstract
The second event of mammalian cell differentiation is the separation between epiblast (EPI) and hypoblast or primitive endoderm (PE). This occurs shortly after segregation of the internal cell mass (ICM) and the trophectoderm (TE). It is known in mice that NANOG acts oppositely to GATA6 and FGF signaling pathway in EPI/PE segregation. Gene expression analyzes group cell populations so tha…