Abstract
It has been assumed that the biosynthesis of Heparan Sulfate ((HS) occurs through a hierarchical sequence of enzymatic events, involving polymerization, sulfation and epimerization, and the 3-O-sulfotransferase (HS3ST) is the last enzyme in this sequence of events. However, we have recently shown that HS3ST can act at different stages of HS biosynthesis and besides enzyme specificity, oth…