Abstract
In our laboratory we obtained parasites expressing subunit alpha1 and alpha 2 as well as the gamma subunit of the AMPK protein kinase of T. cruzi containing the myc peptide sequence using the CRISPR/Cas9 technique. We also found that these subunits are phosphorylated when parasites are incubated with glucose oxidase and glucose to produce hydrogen peroxide. Thus, we intend to immunoprecip…