CV Lattes
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Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP) (Institutional affiliation from the last research proposal) Birthplace: Brazil
Currently I am a postdoctoral fellow at Johns Hopkins University / USA. I work in the area of Microbiology, Biochemistry and Molecular Biology, with an emphasis on Protein Engineering and Synthetic Biology, working on the design and creation of multifunctional enzymes and "Protein switches", by rational design and directed evolution of proteins. My main interests are: Structure and function of proteins, enzymology, regulation of gene expression and synthetic biology. (Source: Lattes Curriculum)
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The evolution of fields as protein engineering and metagenomics depends on the development of tools to exploit, with efficiency, the immense natural diversity in which the target enzymes are immersed. Therefore, to explore this astronomical sample space is necessary to create high throughput screening or selection (HTS) systems more efficient. However, despite the advancement in the const…
The project proposes the use of a new approach for directed evolution. It allows the creation of allosteric proteins by In Vitro Recombination of Nonhomologous Genes. This technique allows to "evolve" enzymes so it can be stimulated by the product, without the need to change the catalytic site, which could lead to a decrease in enzymatic activity. Using a library of random domain insertio…
Conventional cellulose pulp bleaching processes use chemical reagents that create a significant environmental impact, which has gained the paper and celulose industry the reputation of being a major source of fluvial and groundwater pollution. In the present project, we propose to create a novel bifunctional enzyme combining xylanase (EC 3.2.1.8) and laccase (EC 1.10.3.2) activities in a …
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