| Full text | |
| Author(s): |
Azevedo, Aline
[1]
;
Toledo, Juliano S.
[1, 2]
;
Defina, Tania
[1]
;
Pedrosa, Andre L.
[3]
;
Cruz, Angela K.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Fed Minas Gerais, Fac Farm, Dept Anal Clin & Toxicol, Belo Horizonte, MG - Brazil
[3] Univ Fed Triangulo Mineiro, Dept Bioquim Farmacol & Fisiol, Uberaba, MG - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Experimental Parasitology; v. 159, p. 222-226, DEC 2015. |
| Web of Science Citations: | 1 |
| Abstract | |
Leishmania contains two phosphoglycerate kinase (PGK) genes, PGKB and PGKC, which code for the cytosolic and glycosomal isoforms of the enzyme, respectively. Although differences in PGKB and PGKC transcript and protein levels and isoform activities have been well documented, the mechanisms of control of both transcript and protein abundance have not been described to date. To better understand the regulation of Leishmania PGK expression, we investigated the stabilities of both PGK transcripts using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) in combination with transcription and trans-splicing inhibitors. Cells were treated with sinefungin and actinomycin D, and RNA decay kinetics were assessed. In addition, immunoblotting and protein synthesis inhibition by cycloheximide were employed to evaluate protein steady states and degradation. We observed increased stabilities of both PGKB mRNA and protein compared with the glycosomal isoform (PGKC). Our results indicate that both post-transcriptional and post-translational events contribute to the distinct expression levels of the PGKB and PGKC isoforms in Leishmania major. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 99/12403-3 - From the genome to the biology of Leishmania: a comprehensive approach |
| Grantee: | Angela Kaysel Cruz |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 06/50323-7 - Control of gene expression and genetlc plasticity in Leishmania |
| Grantee: | Angela Kaysel Cruz |
| Support Opportunities: | Research Projects - Thematic Grants |