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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the frequency of G-765C polymorphism in the promoter region of the COX-2 gene and its correlation with the expression of this gene in the endometrium of women with endometriosis

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Author(s):
Cavalcanti, Viviane [1] ; Ponce, Tatiana Guida [1] ; Mafra, Fernanda Abani [1] ; Andre, Gustavo Mendonca [1] ; Christofolini, Denise Maria [1] ; Barbosa, Caio Parente [1] ; Bianco, Bianca [1]
Total Authors: 7
Affiliation:
[1] Fac Med ABC, Human Reprod & Genet Ctr, Dept Collect Hlth, BR-09060650 Santo Andre, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ARCHIVES OF GYNECOLOGY AND OBSTETRICS; v. 293, n. 1, p. 109-115, JAN 2016.
Web of Science Citations: 4
Abstract

To evaluate the frequency of polymorphism G-765C (rs20417) of the COX-2 gene and the expression of this gene in the endometrium of women with endometriosis. This is a case-control study of 365 women with endometriosis (251 infertile and 114 fertile) submitted to laparoscopy/laparotomy with histological confirmation of endometriosis. The control group was composed of 522 fertile women without endometriosis. Of these, 37 patients from the endometriosis group and 47 from the control group were submitted to biopsy of the endometrium for analysis of the expression of the COX-2 gene. The genotypes were determined using analysis by High-Resolution Melt. Gene expression was measured by qRT-PCR with TaqMan methodology using the GAPDH gene as normalizer of the reactions. The distribution of the genotypes and alleles in the group of fertile women with moderate/severe endometriosis showed a statistically significant difference, demonstrating association of the ancestral allele, -765G, with increased risk of endometriosis (p = 0.028; OR 0.53; CI 0.32-0.90). The mean expression of the COX-2 gene (mRNA PTGS2) in the group of women with endometriosis was statistically higher compared to the control group (3.85 versus 2.84, p = 0.028). The present study identified that in Brazilian women the presence of the ancestral allele, -765G, of the COX-2 gene is associated with an increased risk for development of moderate/severe endometriosis associated with fertility, and that the eutopic endometrium of women with endometriosis showed increased expression of COX-2 when compared to the control group. (AU)

FAPESP's process: 13/07978-6 - Evaluation of G-765C polymorphism in the promoter region of the COX-2 gene in women with implantation failure and endometriosis
Grantee:Tatiana Guida Ponce
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/03564-0 - Evaluation of the frequency of G-765C polymorphism in the promoter region of the COX2 gene and correlation with the expression of this gene in the endometrium of women with implantation failure with and without endometriosis
Grantee:Bianca Alves Vieira Bianco
Support type: Regular Research Grants