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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histopathological Correlates of Global and Segmental Left Ventricular Systolic Dysfunction in Experimental Chronic Chagas Cardiomyopathy

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Lemos de Oliveira, Luciano Fonseca [1] ; Dias Romano, Minna Moreira [1] ; Vieira de Carvalho, Eduardo Elias [1] ; Cabeza, Jorge Mejia [2] ; Salgado, Helio Cesar [1] ; Fazan Junior, Rubens [1] ; Costa, Renata Sesti [1] ; da Silva, Joao Santana [1] ; Higuchi, Maria de Lourdes [3] ; Maciel, Benedito Carlos [1] ; Cunha-Neto, Edecio [3] ; Marin-Neto, Jose Antonio [1] ; Simoes, Marcus Vinicius [1]
Total Authors: 13
[1] Univ Sao Paulo, Med Sch Ribeirao Preto, BR-14049 Ribeirao Preto - Brazil
[2] Hosp Israelita Albert Einstein, Sao Paulo - Brazil
[3] Univ Sao Paulo, Heart Inst InCor, Fac Med, BR-14049 Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 5

Background-Chronic Chagas cardiomyopathy in humans is characterized by segmental left ventricular wall motion abnormalities (WMA), mainly in the early stages of disease. This study aimed at investigating the detection of WMA and its correlation with the underlying histopathological changes in a chronic Chagas cardiomyopathy model in hamsters. Methods and Results-Female Syrian hamsters (n= 34) infected with 3.59104 or 105 blood trypomastigote Trypanosoma cruzi (Y strain) forms and an uninfected control group (n= 7) were investigated. After 6 or 10 months after the infection, the animals were submitted to in vivo evaluation of global and segmental left ventricular systolic function by echocardiography, followed by euthanasia and histological analysis for quantitative assessment of fibrosis and inflammation with tissue sampling in locations coinciding with the left ventricular wall segmentation employed at the in vivo echocardiographic evaluation. Ten of the 34 infected animals (29%) showed reduced left ventricular ejection fraction (< 73%). Left ventricular ejection fraction was more negatively correlated with the intensity of inflammation (r= -0.63; P< 0.0001) than with the extent of fibrosis (r= -0.36; P= 0.036). Among the 24 animals with preserved left ventricular ejection fraction (82.9 +/- 5.5%), 8 (33%) showed segmental WMA predominating in the apical, inferior, and posterolateral segments. The segments exhibiting WMA, in comparison to those with normal wall motion, showed a greater extent of fibrosis (9.3 +/- 5.7% and 7 +/- 6.3%, P< 0.0001) and an even greater intensity of inflammation (218.0 +/- 111.6 and 124.5 +/- 84.8 nuclei/mm(2), P< 0.0001). Conclusions-Isolated WMA with preserved global systolic left ventricular function is frequently found in Syrian hamsters with experimental chronic Chagas cardiomyopathy whose underlying histopathological features are mainly inflammatory. (AU)

FAPESP's process: 11/03261-4 - Study of the myocardial sympathetic innervation disturbance and myocardial perfusion changes and their correlation with the progression of the left ventricular dysfunction in an experimental model of chronic Changes cardiomyopathy
Grantee:Marcus Vinicius Simões
Support Opportunities: Regular Research Grants