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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes

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Author(s):
Flavia Maziero Andreghetto ; Maria Fatima Guarizo Klingbeil [2] ; Renata Machado Soares [3] ; Roberta Sitnik [4] ; Décio dos Santos Pinto Junior [5] ; Monica Beatriz Mathor [6] ; Fabio Daumas Nunes [7] ; Patricia Severino
Total Authors: 8
Document type: Journal article
Source: Einstein (São Paulo); v. 9, n. 4, p. 442-448, 2011-12-00.
Abstract

ABSTRACT Objective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas. Methods: The expression level of four microRNAs was assessed in cell lines and in primary cultures of oral keratinocytes using specific real-time polymerase chain reactions. The identity of oral squamous cell carcinoma cell lines was confirmed by means of STR (short tandem repeats) profiling. The possible impact of feeder-layer gene expression in global microRNA expression results from keratinocyte primary culture was also evaluated. Results: Significant differences in microRNA gene expression were observed among squamous cell carcinoma cell lines, particularly among cells lines from distinct subsites, as well as between primary culture of human keratinocytes and immortalized keratinocyte cell lines. Conclusions: Primary cultures of human keratinocytes and diverse tumor cell lines are relatively easy to obtain. However, each cell model possesses a characteristic phenotype; whereas one may be useful for a specific study, it may be inappropriate for another. Therefore, it is imperative that suitable cell lines are cautiously selected for functional studies in cancer. (AU)

FAPESP's process: 09/04166-5 - Characterization of microRNA regulatory networks involved in head and neck squamous cell carcinoma by means of microarray and functional studies in cell culture
Grantee:Patricia Severino
Support type: Regular Research Grants