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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene expression of thyroid-specific transcription factors may help diagnose thyroid lesions but are not determinants of tumor progression

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Author(s):
Batista, F. A. [1] ; Ward, L. S. [1] ; Marcello, M. A. [1] ; Martins, M. B. [1] ; Peres, K. C. [1] ; Torricelli, C. [1] ; Bufalo, N. E. [1] ; Soares, F. A. [2] ; da Silva, M. J. [3] ; Assumpcao, L. V. M. [1]
Total Authors: 10
Affiliation:
[1] Univ Campinas Unicamp, Fac Med Sci FCM, Lab Canc Mol Genet, Sch Med Sci, Rua Tessalia Vieira de Camargo 126, BR-13083887 Sao Paulo - Brazil
[2] AC Camargo Hosp, Antonio Prudente Fdn, Dept Pathol, Rua Prof Antonio Prudente 211, BR-01509010 Sao Paulo - Brazil
[3] Univ Campinas Unicamp, CBMEG, Ave Candido Rondon, Cidade Univ Zeferino Vaz, BR-13083875 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Endocrinological Investigation; v. 39, n. 4, p. 423-429, APR 2016.
Web of Science Citations: 6
Abstract

Purpose The role of thyroid-specific transcription factors in thyroid malignancy is still poorly understood, so we investigate thyroid-specific transcription factors gene expression both in benign and in malignant thyroid nodules, aiming to study a possible clinical utility of these molecules. Methods We quantified TTF-1, FOXE1 and PAX8 mRNA levels, relating their expression to diagnostic and prognostic features of thyroid tumors. RNA was extracted from 4 normal thyroid tissues, 101 malignant {[}99 papillary thyroid carcinomas (PTC) and 2 anaplastic thyroid carcinomas] and 99 benign thyroid lesion tissues {[}49 goiter and 50 follicular adenomas (FA)]. Results Levels of mRNA of both FOXE1 (P < 0.0001) and PAX8 (P < 0.0001) genes, but not TTF-1 (P = 0.7056), were higher in benign than in malignant thyroid lesions. FOXE1 was able to identify malignant nodules with 75.8 % sensitivity, 76.1 % specificity, 75.8 % positive predictive value, 76.1 % negative predictive value and 75.9 % accuracy. PAX8 was able to identify malignancy with 60.6 % sensitivity, 81.1 % specificity, 76.9 % positive predictive value, 66.4 % negative predictive value and 70.6 % accuracy. Both FOXE1 and PAX8 gene expression patterns were also able to differentiate FA from the follicular variant of PTC-FVPTC. However, the investigated gene expression was neither associated with any clinical feature of tumor aggressiveness nor associated with recurrence or survival. Conclusions We suggest that FOXE1 and PAX8 gene expression patterns may help to diagnose thyroid nodules, identifying malignancy and characterizing follicular-patterned thyroid lesions, but are not determinants of thyroid tumor progression. (AU)

FAPESP's process: 13/18683-7 - Study of transcription factors and their relation with thyroid nodules
Grantee:Ligia Vera Montali da Assumpção
Support type: Regular Research Grants