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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biophysical Characterization of the Nucleoside Diphosphate Kinase of Leishmania major and Effect of the P95S Mutation

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Vieira, Plinio S. [1, 2] ; de Jesus Santos, Ana P. [1] ; de Oliveira, Arthur H. C. [1]
Total Authors: 3
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto FF, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
[2] CNPEM, LNBio, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PROTEIN AND PEPTIDE LETTERS; v. 23, n. 2, p. 99-106, 2016.
Web of Science Citations: 0

Nucleoside diphosphate kinases (NDK; EC are enzymes required for maintaining intracellular levels of nucleosides triphosphates (NTP) through transfer the gamma- phosphoryl group from a NTP to a NDP. The enzyme is associated with several biological functions including prevention of host ATP-mediated cytolysis during pathogenic infections. Here we present the biophysical characterization of NDK from Leishmania major and the effect of a mutation on the protein structure in solution. The structural stability was analyzed since this secreted protein may act in different microenvironments at various stages of the parasite life cycle. LmNDK and P95S mutant were subjected to denaturation with pH and guanidine. Structural transitions were monitored by circular dichroism and intrinsic fluorescence tryptophan emission. Our results showed that the LmNDK is more structurally stable than other described NDKs and that the catalytically active P95S mutant in the Kpn loop presented a decrease in protein stability, indicating the importance of this proline for maintenance of the LmNDK structure. (AU)

FAPESP's process: 11/20569-2 - Role of amino acids of the active site and interface in oligomeric in the structure and function of the nucleoside diphosphate kinase of Leishmania major
Grantee:Arthur Henrique Cavalcante de Oliveira
Support type: Regular Research Grants
FAPESP's process: 07/06755-2 - Structural and functional characterization of proteins envolved in the virulence of the protozoan parasite Leishmania major
Grantee:Arthur Henrique Cavalcante de Oliveira
Support type: Regular Research Grants
FAPESP's process: 10/03761-4 - Kinetic characterization and effect of mutations in the oligomeric interface of the Nucleoside Diphosphate Kinase from Leishmania major.
Grantee:Plínio Salmazo Vieira
Support type: Scholarships in Brazil - Master