Triiodothyronine (T-3) induces HIF1A and TGFA expression in MCF7 cells by activating PI3K

Author(s)

Fontes Moretto, Fernanda Cristina ^[1] ; De Sibio, Maria Teresa ^[1] ; Luvizon, Aline Carbonera ^[2] ; Castro Olimpio, Regiane Marques ^[1] ; de Oliveira, Miriane ^[1] ; Barnabe Alves, Carlos Augusto ^[3] ; Conde, Sandro Jose ^[1] ; Nogueira, Celia Regina ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Biosci Inst, Dept Morphol, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 154, p. 52-57, JUN 1 2016.
• Web of Science Citations: 3

Abstract

High expression levels of hypoxia inducing factor 1 alpha are related to mammary carcinogenesis. In previous studies, we demonstrated that expression of transforming growth factor alpha increases upon treatment with triiodothyronine, but this expression does not occur in cellular models that do not express the estrogen receptor, or when cells are co-treated with the anti-estrogen, tamoxifen. The aim of this study was to determine the effect of the hormone triiodothyronine on the expression of the genes HIF1A and TGFA in the breast cancer cell line MCF7. The cell line was subjected to treatment with triiodothyronine at the supraphysiological dose of 10(-8) M for 10 min, 30 min, 1 h, and 4 h in the presence or absence of actinomycin D, the gene expression inhibitor, cycloheximide, the protein synthesis inhibitor, and LY294002, the phosphoinositide 3 kinase inhibitor. HIF1A and TGFA mRNA expression was analyzed by reverse transcription polymerase chain reaction. For data analysis, we used analysis of variance complemented by Tukey test and an adopted minimum of 5% significance. We found that HIF1A and TGFA expression increased in the presence of triiodothyronine at all times studied. HIF1A expression decreased in triiodothyronine-treated cells when gene transcription was also inhibited; however, TGFA expression decreased after 10 and 30 min of treatment even when transcription was not inhibited. We found that activation of PI3K was necessary for triiodothyronine to modulate HIF1A and TGFA expression. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/15209-5 - PARTICIPATION OF T3 IN POST-TRANSCRIPTIONAL CONTROL OF HIF-1± AND TGF± EXPRESSION IN CELL LINES OF BREAST CANCER AND EVALUATION OF THE INVOLVEMENT OF POTENTIAL miRNAS
• Grantee: Fernanda Cristina Fontes Moretto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/04684-9 - Extranuclear actions of triiodothyronine and estrogen on breast cancer cell lines
• Grantee: Celia Regina Nogueira
• Support Opportunities: Regular Research Grants