In vitro culture of bovine steroidogenic luteal cells and non-steroidogenic. effec...
| Full text | |
| Author(s): |
Destro, F. C.
;
Martin, I.
;
Landim-Alvarenga, F. D. C.
;
Ferreira, J. C. P.
;
Pate, J. L.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | REPRODUCTION IN DOMESTIC ANIMALS; v. 51, n. 5, p. 848-852, OCT 2016. |
| Web of Science Citations: | 0 |
| Abstract | |
The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid-luteal stage (at 10-12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 mu g/ml); LH (100 mu g/ml); CONA + LH; LH (100 mu g/ml) + prostaglandin F2 alpha (PGF2 alpha) (10 ng/ml); CONA + LH + PGF2 alpha. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p <.05. Culture in the presence of CONA decreased the P4-secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments. (AU) | |
| FAPESP's process: | 13/07439-8 - In vitro culture of cells from bovine corpus luteum for the study of the events associated with luteolysis |
| Grantee: | João Carlos Pinheiro Ferreira |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/01940-2 - In vitro culture of bovine steroidogenic and non-steroidogenic luteal cells. effect of PGF2a on the expression of genes and proteins associated with steroidogenesis and apoptosis |
| Grantee: | Flavia Caroline Destro |
| Support type: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/00992-3 - In vitro culture of bovine steroidogenic luteal cells and non-steroidogenic. effect of PGF2a on the expression of genes and proteins associated with steroidogenesis and apoptosis |
| Grantee: | Flavia Caroline Destro |
| Support type: | Scholarships in Brazil - Doctorate |