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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro

Full text
Author(s):
Destro, F. C. ; Martin, I. ; Landim-Alvarenga, F. D. C. ; Ferreira, J. C. P. ; Pate, J. L.
Total Authors: 5
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 51, n. 5, p. 848-852, OCT 2016.
Web of Science Citations: 0
Abstract

The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid-luteal stage (at 10-12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 mu g/ml); LH (100 mu g/ml); CONA + LH; LH (100 mu g/ml) + prostaglandin F2 alpha (PGF2 alpha) (10 ng/ml); CONA + LH + PGF2 alpha. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p <.05. Culture in the presence of CONA decreased the P4-secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments. (AU)

FAPESP's process: 13/07439-8 - In vitro culture of cells from bovine corpus luteum for the study of the events associated with luteolysis
Grantee:João Carlos Pinheiro Ferreira
Support Opportunities: Regular Research Grants
FAPESP's process: 15/01940-2 - In vitro culture of bovine steroidogenic and non-steroidogenic luteal cells: effect of PGF2a on the expression of genes and proteins associated with steroidogenesis and apoptosis
Grantee:Flavia Caroline Destro
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/00992-3 - In vitro culture of bovine steroidogenic luteal cells and non-steroidogenic. Effect of PGF2a on the expression of genes and proteins associated with steroidogenesis and apoptosis
Grantee:Flavia Caroline Destro
Support Opportunities: Scholarships in Brazil - Doctorate