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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the universal stress protein F from atypical enteropathogenic Escherichia coli and its prevalence in Enterobacteriaceae

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Author(s):
de Souza, Cristiane S. ; Torres, Alfredo G. ; Caravelli, Andressa ; Silva, Anderson ; Polatto, Juliana M. ; Piazza, Roxane M. F.
Total Authors: 6
Document type: Journal article
Source: Protein Science; v. 25, n. 12, p. 2142-2151, DEC 2016.
Web of Science Citations: 4
Abstract

Atypical enteropathogenic Escherichia coli (aEPEC) are heterogeneous strains in terms of serotypes, adherence patterns and the presence of novel virulence factors. This heterogeneity is intriguing, promoting studies trying to characterize these novel proteins and to better comprehend this pathotype group. In a previous study analyzing low-molecular mass proteomes of four representative aEPEC strains of three different adhesion phenotypes, we classified proteins according to their annotated function, with most of them being involved in metabolism and transport; while some of them were classified as hypothetical proteins. The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. One of the hypothetical proteins was annotated as Z2335, with orthologue in EPEC, and by bioinformatics analysis, this protein was revealed to be the universal stress protein F (UspF). Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a alpha/beta, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae. (AU)

FAPESP's process: 09/14845-7 - Immunodiagnosis of diarrheagenic Escherichia coli
Grantee:Roxane Maria Fontes Piazza
Support type: Regular Research Grants
FAPESP's process: 08/09206-2 - Clonig, expression and features analysis of proteins of Escherichia coli enteropathogenic atypical (EPEC atypical) of serogroup O55:H7
Grantee:Cristiane Santos de Souza
Support type: Scholarships in Brazil - Post-Doctorate