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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification and characterization of microRNAs in the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria (Diptera: Calliphoridae)

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Author(s):
Paulo, D. F. ; Azeredo-Espin, A. M. L. ; Canesin, L. E. C. ; Vicentini, R. ; Junqueira, A. C. M.
Total Authors: 5
Document type: Journal article
Source: Insect Molecular Biology; v. 26, n. 1, p. 46-57, FEB 2017.
Web of Science Citations: 1
Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression through post-transcriptional regulation. Here, we report the identification and characterization of miRNAs in two closely related screw-worm flies with different feeding habits: Cochliomyia hominivorax and Cochliomyia macellaria. The New World screwworm, C. hominivorax, is an obligatory parasite of warm-blooded vertebrates, whereas the secondary screwworm, C. macellaria, is a free-living organism that feeds on decaying organic matter. Here, the small RNA transcriptomes of adults and third-instar larvae of both species were sequenced. A total of 110 evolutionarily conserved miRNAs were identified, and 10 putative precursor miRNAs (pre-miRNAs) were predicted. The relative expression of six selected miRNAs was further investigated, including miRNAs that are related to reproduction and neural processes in other insects. Mature miRNAs were also characterized across an evolutionary time scale, suggesting that the majority of them have been conserved since the emergence of the Arthropoda {[}540 million years ago (Ma)], Hexapoda (488 Ma) and Brachycera (195 Ma) lineages. This study is the first report of miRNAs for screwworm flies. We also performed a comparative analysis with the hereby predicted miRNAs from the sheep blowfly, Lucilia cuprina. The results presented may advance our understanding of parasitic habits within Calliphoridae and assist further functional studies in blowflies. (AU)

FAPESP's process: 12/11654-9 - Insecticide resistance characterization in the New World screwworm fly, Cochliomyia hominivorax: molecular and populational approaches
Grantee:Ana Maria Lima de Azeredo-Espin
Support Opportunities: Regular Research Grants
FAPESP's process: 12/06272-0 - Identification and characterization of microRNAs in Cochliomyia hominivorax and Cochliomyia macellaria (Diptera: Calliphoridae)
Grantee:Daniel Fernando Paulo
Support Opportunities: Scholarships in Brazil - Master