Effect of temperature on sugarcane ethanol fermentation: Kinetic modeling and validation under very-high-gravity fermentation conditions

Author(s)

Rivera, Elmer Ccopa ; Yamakawa, Celina K. ; Saad, Marcelo B. W. ; Atala, Daniel I. P. ; Ambrosio, Wesley B. ; Bonomi, Antonio ; Nolasco Junior, Jonas ; Rossell, Carlos E. V.

• Document type: Journal article
• Source: Biochemical Engineering Journal; v. 119, p. 42-51, MAR 15 2017.
• Web of Science Citations: 10

Abstract

In this work, a mechanistic model is developed to simulate the effect of temperature on Saccharomyces cerevisiae growth and ethanol production of batch fermentations. A wide temperature range is used to estimate the temperature-dependent kinetic parameters of the reaction kinetics. Because multi parameter estimation problems are complex, an optimization-based procedure is used to determine the optimum parameter values. The calculated reaction rates are used to construct a mechanistic fed-batch model. Experimental data from several cycles of very-high-gravity (VHG) ethanol fermentation from sugarcane are used to validate the model. Acceptable predictions are achieved in terms of the residual standard deviation (RSD). In addition, a suitable fermentation temperature profile, nutrient supplementation and micro-aeration during cell treatment are essential factors to obtain a yield of up to 90%, with a productivity of 10.2 g/L h and an ethanol concentration of 120 g/L. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/01785-0 - Butanol production from eucalyptus wood: effect of glycerol on in situ detoxification of lignocellulose-derived microbial inhibitors and in silico analysis of the metabolism of Clostridium beijerinckii NCIMB 8052
• Grantee: Elmer Alberto Ccopa Rivera
• Support Opportunities: Scholarships abroad - Research