| Full text | |
| Author(s): |
Mendonca, Thatiane T.
;
Tavares, Rafaela R.
;
Cespedes, Lucas G.
;
Sanchez-Rodriguez, Ruben J.
;
Schripsema, Jan
;
Taciro, Marilda K.
;
Gomez, Jose G. C.
;
Silva, Luiziana F.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 98, p. 654-663, MAY 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Biopolymers as polyhydroxyalkanoates (PHA) composed by different co-monomers 3-hydroxybutyrate and 3-hydroxyhexanoate {[}P(3 HB-co-3HHx)] has attracted interest since its properties are similar to low density polyethylene. Burkholderia sacchari produces this copolymer with a very low3 HHx molar fraction, about 2 mol%. B. sacchari mutant (unable to produce polymer) was engineered to host PHA biosynthesis genes (phaPCJ) from Aeromonas sp. In addition, a two-step bioprocess to increase biopolymer production was developed. The combination of these techniques resulted in the production of P(3HB-co-3HHx) with 3HHx content up to 20 mol%. The PHA content was about 78% of dry biomass, resulting in PHA volumetric productivities around 0.45 gl(-1) h (-1). The P(3HB-co-3HHx) containing 20 mol% of 3HHx presented an elongation at brake of 945%, higher than reported before for this PHA composition. Here we have described an approach to increase 3HHx content into the copolymer, allowing the precise control of the 3HHx molar fractions. (C) 2017 Elsevier rights reserved. (AU) | |
| FAPESP's process: | 12/51533-6 - Tailor-made biodegradable polymers produced ·by bacteria from biomass |
| Grantee: | Luiziana Ferreira da Silva |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |