RNA-Seq of bovine preipheral blood leukocytes: functional analysis of the transcri...
| Full text | |
| Author(s): Show less - |
Maruyama, Sandra R.
;
Garcia, Gustavo R.
;
Teixeira, Felipe R.
;
Brandao, Lucinda G.
;
Anderson, Jennifer M.
;
Ribeiro, Jose M. C.
;
Valenzuela, Jesus G.
;
Horackova, Jana
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Verissimo, Ceclia J.
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Katiki, Luciana M.
;
Banin, Tamy M.
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Zangirolamo, Amanda F.
;
Gardinassi, Luiz G.
;
Ferreira, Beatriz R.
;
de Miranda-Santos, Isabel K. F.
Total Authors: 15
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| Document type: | Journal article |
| Source: | PARASITES & VECTORS; v. 10, APR 26 2017. |
| Web of Science Citations: | 8 |
| Abstract | |
Background: Ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used to control infestations. To guide antigen discovery for a vaccine that targets the gamut of parasitic strategies mediated by tick saliva and enables immunological memory, we exploited a transcriptome constructed from salivary glands from all stages of Rhipicephalus microplus ticks feeding on genetically tick-resistant and susceptible bovines. Results: Different levels of host anti-tick immunity affected gene expression in tick salivary glands; we thus selected four proteins encoded by genes weakly expressed in ticks attempting to feed on resistant hosts or otherwise abundantly expressed in ticks fed on susceptible hosts; these sialoproteins mediate four functions of parasitism deployed by male ticks and that do not induce antibodies in naturally infected, susceptible bovines. We then evaluated in tick-susceptible heifers an alum-adjuvanted vaccine formulated with recombinant proteins. Parasite performance (i.e. weight and numbers of females finishing their parasitic cycle) and titres of antigen-specific antibodies were significantly reduced or increased, respectively, in vaccinated versus control heifers, conferring an efficacy of 73.2%; two of the antigens were strong immunogens, rich in predicted T-cell epitopes and challenge infestations boosted antibody responses against them. Conclusion: Mining sialotranscriptomes guided by the immunity of tick-resistant hosts selected important targets and infestations boosted immune memory against salivary antigens. (AU) | |
| FAPESP's process: | 12/15464-0 - RNA-Seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle |
| Grantee: | Sandra Regina Costa Maruyama |
| Support type: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 12/04087-0 - RNA-seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle |
| Grantee: | Sandra Regina Costa Maruyama |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 10/16464-8 - Application of protein microarray to identify substrates of SCF1 (FBXO25) E3 ubiquitin ligase |
| Grantee: | Felipe Roberti Teixeira |
| Support type: | Scholarships in Brazil - Post-Doctorate |