Novel Selectivity-Based Forensic Toxicological Validation of a Paper Spray Mass Spectrometry Method for the Quantitative Determination of Eight Amphetamines in Whole Blood

Author(s)

Teunissen, Sebastiaan F. ^{[1, 2, 3]} ; Fedick, Patrick W. ^{[1, 3]} ; Berendsen, Bjorn J. A. ^[4] ; Nielen, Michel W. F. ^{[4, 5]} ; Eberlin, Marcos N. ^[2] ; Cooks, R. Graham ^{[1, 3]} ; van Asten, Arian C. ^{[6, 7, 8]}

Affiliation

^[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 - USA

^[2] Univ Campinas Unicamp, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

^[3] Purdue Univ, Ctr Analyt Instrumentat, W Lafayette, IN 47907 - USA

^[4] Wageningen Res, RIKILT, Akkermaalsbos 2, 6708 WB, POB 230, NL-6700 AE Wageningen - Netherlands

^[5] Wageningen Univ, Lab Organ Chem, Stippeneng 4, NL-6708 WE Wageningen - Netherlands

^[6] Netherlands Forens Inst, POB 24044, NL-2490 AA The Hague - Netherlands

^[7] Univ Amsterdam, Vant Hoff Inst Mol Sci, Fac Sci, POB 94157, NL-1090 GD Amsterdam - Netherlands

^[8] Amsterdam Ctr Forens Sci & Med, CLHC, POB 94157, NL-1090 GD Amsterdam - Netherlands

• Document type: Journal article
• Source: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 28, n. 12, p. 2665-2676, DEC 2017.
• Web of Science Citations: 13

Abstract

Paper spray tandem mass spectrometry is used to identify and quantify eight individual amphetamines in whole blood in 1.3 min. The method has been optimized and fully validated according to forensic toxicology guidelines, for the quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), para-methoxyamphetamine (PMA), para-methoxymethamphetamine (PMMA), and 4-fluoroamphetamine (4-FA). Additionally, a new concept of intrinsic and application-based selectivity is discussed, featuring increased confidence in the power to discriminate the amphetamines from other chemically similar compounds when applying an ambient mass spectrometric method without chromatographic separation. Accuracy was within +/- 15% and average precision was better than 15%, and better than 20% at the LLOQ. Detection limits between 15 and 50 ng/mL were obtained using only 12 mu L of whole blood. (AU)

• FAPESP's process: 16/01683-2 - Development and full validation of qualitative and quantitative ambient mass spectrometry based methods applied in forensic chemistry
• Grantee: Marcos Nogueira Eberlin
• Support Opportunities: Research Grants - Visiting Researcher Grant - International