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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel Hybrid of Typical Enteropathogenic Escherichia coli and Shiga-Toxin-Producing E-coli (tEPEC/STEC) Emerging From Pet Birds

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Author(s):
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Gioia-Di Chiacchio, Rosely Martins [1, 2] ; Vieira Cunha, Marcos Paulo [1] ; Marques de Sa, Lilian Rose [1] ; Davies, Yame Minieiro [1] ; Pacheco Pereira, Camila Bueno [3] ; Martins, Fernando Henrique [3] ; Munhozs, Danielle Dias [3] ; Abe, Cecilia Mari [3] ; Franzolin, Marcia Regina [3] ; dos Santos, Luis Fernando [4] ; Cabilio Guth, Beatriz Ernestina [5] ; Elias, Waldir Pereira [3] ; Fontes Piazza, Roxane Maria [3] ; Knobl, Terezinha [1]
Total Authors: 14
Affiliation:
[1] Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil
[2] Univ Paulista, Sch Vet Med, Sao Paulo - Brazil
[3] Butantan Inst, Sao Paulo - Brazil
[4] Adolfo Lutz Inst, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 9, DEC 6 2018.
Web of Science Citations: 1
Abstract

Exotic psittacine birds have been implicated as reservoir of diarrheagenic Escherichia coil (E. coli), including enteropathogenic E. coli (EPEC) and Shiga-toxin producing E coli (STEC). Here, we present a genotypic and phenotypic characterization of typical EPEC/STEC hybrid strains isolated from exotic psittacine birds. The strains were positive for eae, bfpA, and stx2f genes, belong to serotype O137:H6 and ST2678. Two strains were subject to whole genome sequencing, confirming the presence of the virulence factors of both E. coli pathotypes. Phenotypical in vitro tests confirmed their ability to adhere to HeLa cells and cause cytotoxicity to Vero cells. The rabbit ileal loop assays showed the attaching and effacing lesion, in addition to inflammatory process and overproduction of intestinal mucus. This is the first report of hybrid typical EPEC/STEC (0137:H6/ST2678) strains isolated from companion psittacine birds and the results suggest zoonotic risks. (AU)

FAPESP's process: 16/12641-9 - Antimicrobial resistance of Escherichia coli isolated from broilers raised in conventional and organic poultry farms
Grantee:Terezinha Knöbl
Support type: Regular Research Grants
FAPESP's process: 13/06589-6 - Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility
Grantee:Roxane Maria Fontes Piazza
Support type: Regular Research Grants