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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fasting promotes functional changes in liver mitochondria

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Menezes-Filho, Sergio L. [1] ; Amigo, Ignacio [1] ; Luevano-Martinez, Luis Alberto [2] ; Kowaltowski, Alicia J. [1]
Total Authors: 4
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS; v. 1860, n. 2, p. 129-135, FEB 1 2019.
Web of Science Citations: 0

Overnight fasting of rodents is commonly adopted in protocols to obtain isolated liver mitochondria, but the effects of fasting itself on mitochondrial function are poorly characterized. In this study we show that overnight fasting (15 h) promotes a shift in the liver mitochondrial bioenergetic profile, with a reduction in ADP-stimulated and maximal respiration, lower membrane potentials and lower resistance to Ca2+-induced mitochondrial permeability transition. Short term fasting (4 h) promoted similar changes, suggesting that this is a physiological shift in mitochondrial function associated with fasting, but not torpor. Our results suggest that the widely adopted liver mitochondrial isolation technique using fasted animals should be reconsidered, and also uncover physiological changes in bioenergetic function associated to nutritional status. (AU)

FAPESP's process: 16/12999-0 - Evolution of the cardiolipin biosynthetic pathway: biochemical implications for the evolution of the Eukarya domain
Grantee:Luis Alberto Luevano Martinez
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC