Triiodothyronine (T-3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7

Author(s):Show less -

Silva, Tabata M. ^[1] ; Moretto, Fernanda C. F. ^[1] ; De Sibio, Maria T. ^[1] ; Goncalves, Bianca M. ^[1] ; Oliveira, Miriane ^[1] ; Olimpio, Regiane M. C. ^[1] ; Oliveira, Diego A. M. ^[2] ; Costa, Sarah M. B. ^[1] ; Depra, Igor C. ^[1] ; Namba, Vickeline ^[1] ; Nunes, Maria T. ^[3] ; Nogueira, Celia R. ^[1]

Affiliation

^[1] Univ Estadual Paulista, Fac Med Botucatu, Dept Med Interna, UNESP, Av Prof Mario Rubens Guimaraes Montenegro S-N, BR-18618687 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 63, n. 2, p. 142-147, MAR-APR 2019.
• Web of Science Citations: 1

Abstract

Objective: To verify the physiological action of triiodothyronine T-3 on the expression of transforming growth factor alpha (TGFA) mRNA in MCF7 cells by inhibition of RNA Polymerase II and the MAPK/ERK pathway. Materials and methods: The cell line was treated with T-3 at a physiological dose (10(-9) M) for 10 minutes, 1 and 4 hour (h) in the presence or absence of the inhibitors, a-amanitin (RNA polymerase II inhibitor) and PD98059 (MAPK/ERK pathway inhibitor). TGFA mRNA expression was analyzed by RT-PCR. For data analysis, we used ANOVA, complemented with the Tukey test and Student t-test, with a minimum significance of 5%. Results: T-3 increases the expression of TGFA mRNA in MCF7 cells in 4 h of treatment. Inhibition of RNA polymerase II modulates the effect of T-3 treatment on the expression of TGFA in MCF7 cells. Activation of the MAPK/ERK pathway is not required for; to affect the expression of TGFA mRNA. Conclusion: Treatment with a physiological concentration of T-3 after RNA polymerase II inhibition altered the expression of TGFA. Inhibition of the MAPK/ERK pathway after T-3 treatment does not interfere with the TGFA gene expression in a breast adenocarcinoma cell line. (AU)

• FAPESP's process: 15/09686-8 - Mechanism of action of triiodothyronine hornone (T3) in the TGFa gene expression in breast adenocarcinoma cell lines
• Grantee: Tabata Marinda da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/15209-5 - PARTICIPATION OF T3 IN POST-TRANSCRIPTIONAL CONTROL OF HIF-1± AND TGF± EXPRESSION IN CELL LINES OF BREAST CANCER AND EVALUATION OF THE INVOLVEMENT OF POTENTIAL miRNAS
• Grantee: Fernanda Cristina Fontes Moretto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives
• Grantee: Maria Tereza Nunes
• Support Opportunities: Research Projects - Thematic Grants