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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterizing the Bioluminescence of the Humboldt Squid, Dosidicus gigas (d'Orbigny, 1835): One of the Largest Luminescent Animals in the World

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Author(s):
Galeazzo, Gabriela A. [1] ; Mirza, Jeremy D. [1, 2] ; Dorr, Felipe A. [3] ; Pinto, Ernani [3] ; Stevani, V, Cassius ; Lohrmann, Karin B. [4] ; Oliveira, Anderson G. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Oceanog, Dept Oceanog Fis Quim & Geol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Quim, Diadema, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo, SP - Brazil
[4] Univ Catolica Norte, Fac Ciencias Mar, Ctr Innovac Acuicola Aquapacifico, Dept Biol Marina, Coquimbo - Chile
Total Affiliations: 4
Document type: Journal article
Source: Photochemistry and Photobiology; v. 95, n. 5, p. 1179-1185, SEP 2019.
Web of Science Citations: 0
Abstract

Bioluminescence is found in a number of cephalopods, such as Watasenia scintillans and Sthenoteuthis oualaniensis; however, many species remain poorly studied, including the Humboldt squid, Dosidicus gigas. This is the largest member of the Ommastrephidae family and grows to 2 m in length, making it one of the largest luminescent animals ever observed. Humboldt squid have small photophores all over their body that emit a brilliant blue luminescence. Using lyophilized photophores from squid caught off the coast of Chile, experiments were conducted to isolate the luciferin and protein involved in its bioluminescence. Methanolic extracts of the photophores were shown to contain dehydrocoelenterazine, and a membrane-bound photoprotein was shown to be involved. This photoprotein was purified using ion exchange chromatography, and SDS-PAGE showed a clean band of approximately 60 kDa. The excised band was analyzed by LC/MS, and the obtained data were compared against the transcriptome data of D. gigas, allowing us to find two gene products which displayed high coverage (>80%), the enzymes symplectin and vanin-2, which potentially associate with light emission process in this organism. Finally, the purified photoprotein was shown to emit a blue light (470 nm) in the presence of dehydrocoelenterazine. (AU)

FAPESP's process: 17/26279-2 - Studies of Chaetopterus variopedatus bioluminescence and new marine luminous systems
Grantee:Anderson Garbuglio de Oliveira
Support type: Regular Research Grants
FAPESP's process: 14/18541-0 - Chaetopterus variopedatus bioluminescence: luciferase purification and ecological roles
Grantee:Jeremy Darius Mirza
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/07677-7 - Bioluminescence in Dosidicus gigas: cloning and purification of a new photoprotein
Grantee:Gabriela Amancio Galeazzo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark
Grantee:Etelvino José Henriques Bechara
Support type: Research Projects - Thematic Grants