Proinflammatory and regulatory mechanisms in allergic contact dermatitis caused by methylchloroisothiazolinone and methylisothiazolinone

Author(s):Show less -

de Oliveira Goes, Heliana Freitas ^{[1, 2]} ; Virgens, Anangelica Rodrigues ^{[1, 2]} ; de Carvalho, Gabriel Costa ^[1] ; Pietrobon, Anna Julia ^{[1, 3]} ; Calvielli Castelo Branco, Anna Claudia ^{[1, 3]} ; da Silva Oliveira, Luanda Mara ^[1] ; Fernandes, Iara G. ^{[1, 2]} ; Pereira, Naiura Vieira ^[4] ; Sotto, Mirian Nacagami ^[4] ; Silva dos Reis, Vitor Manoel ^[2] ; Sato, Maria Notomi ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Dept Dermatol, Lab Dermatol & Immunodeficiencies, Med Sch, LIM 56, Av Dr Eneas de Carvalho Aguiar 470, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Dermatol, Med Sch, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sciencies, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Pathol, Med Sch, Sao Paulo - Brazil

• Document type: Journal article
• Source: EXPERIMENTAL DERMATOLOGY; v. 29, n. 5 APR 2020.
• Web of Science Citations: 0

Abstract

Background Methylchloroisothiazolinone (MCI) and methylisothiazolinone (MI) are the cause of an increasing number of contact allergies. Understanding the mechanisms by which MCI/MI induces proinflammatory and regulatory factors production is necessary to understand the outcome of allergic contact dermatitis (ACD). Objectives To evaluate the dysfunction of proinflammatory cytokines and regulatory factors in the positive MCI/MI patch test at the transcriptional and protein expression levels. Moreover, to analyse the cytokines production induced by MI in peripheral blood mononuclear cells (PBMCs). Materials and Methods The selected patients had positive MCI/MI patch test results. The expression of proinflammatory factors was evaluated by q-PCR and immunochemistry at 48 hours of positive MCI/MI patch test. The MCI/MI- or MI- induced secretion of IL-1 beta, TNF and IL-6 by PBMC was analysed by flow cytometry. Results The results showed a decreased TLR4 expression with upregulated IL6, FOXP3, IL10 and TGF beta mRNA expression as assessed by q-PCR at the site of the MCI/MI skin reaction. We detected increased protein levels of TLR4, FOXP3 and IL-10 in the dermis layer in the ACD reaction by immunocitochemistry. Moreover, MCI/MI induced proinflammatory cytokine production by PBMC through the NF-kappa B signalling pathway. Conclusion Considering the altered innate immune response triggered by MCI/MI sensitization, these findings indicate that the regulatory process at the induction phase of ACD is a crucial mechanism. Given the increase in occupational and domestic exposure to MCI/MI, the underlying immunological mechanisms should be understood. (AU)

• FAPESP's process: 17/12475-4 - Analysis of the pattern recognition receptors and crosstalk between dendritic cells-T lymphocytes and macrophages in allergic contact dermatitis by methylisothiazolinone and methylchloroisothiazolinone
• Grantee: vitor manoel silva dos reis
• Support Opportunities: Regular Research Grants