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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Correlation of plasma butyrylcholinesterase concentration with Acethylcholinesterase H353N polymorphism in the inflammatory response of Chagas disease patients

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Author(s):
Campello, A. C. [1] ; Zanella, L. G. F. A. B. D. E. [2, 3] ; Suzuki, R. B. [1, 4] ; Tokumo, M. O. [5] ; Chagas, E. F. B. [6] ; Baleotti, W. [7] ; Speranca, M. A. [8] ; Martins, L. P. A. [1]
Total Authors: 8
Affiliation:
[1] Marilia Med Sch, Dept Parasitol, Monte Carmelo Ave 800, BR-17519030 Marilia, SP - Brazil
[2] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo, SP - Brazil
[3] Servidor Publ Hosp, BR-04038034 Sao Paulo, SP - Brazil
[4] Marilia Univ, Sch Med, BR-17525902 Marilia, SP - Brazil
[5] Marilia Med Sch, Dept Cardiol, BR-17519030 Marilia, SP - Brazil
[6] Marilia Univ, Study Grp Aging & Obes GEEO, BR-17525902 Marilia, SP - Brazil
[7] Marilia Med Sch, Dept Clin Pathol, BR-17519030 Marilia, SP - Brazil
[8] Fed Univ ABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Parasitology International; v. 76, JUN 2020.
Web of Science Citations: 0
Abstract

Trypanosoma cruzi infection triggers an intense production of pro-inflammatory cytokines mediated by T helper 1 lymphocytes, inducing the anti-inflammatory reflex of acetylcholine (ACh). The ACh concentration modulation is associated to the two major esterases, the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). AChE H353N protein polymorphism is related to low Chagas chronic disease prognostic. In order to evaluate the correlation of plasmatic BuChE concentration and the presence of AChE H353N polymorphism in Chagas disease patients and healthy individuals, we studied two groups of individuals, one of 61 Chagas disease patients and another of 74 healthy individuals. Plasma concentration of BuChE was measured by the chemiluminescent method and AChE H353N polymorphism was investigated by PCR-RFLP and sequencing of the respective encoding AChE gene fragment. The BuChE concentration was statistically higher in Chagas disease patients, with no AChE genotype significant influence. AChE genotypes YT{*}A/YT{*}A, YT{*}A/YT{*}B and YT{*}B/YT{*}B, respectively, were expressed in 53 (86.88%), 7 (11.46%) and one (1.64%) chagasic patients, and in 68 (91.89%), 6 (8.10%) and none healthy individuals. BuChE activity may represent an important marker for chronic Chagas disease inflammatory process and prognostic. Lower BuChE concentration correlated with AChE YT{*}B allele, although without statistical power. (AU)

FAPESP's process: 11/23378-3 - Study of colon contractile response in infected mice with blood trypomastigotes forms of Trypanosoma cruzi QM2 strain
Grantee:Luiz Gonzaga Francisco de Assis Barros D'Elia Zanella
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/14166-8 - Research for mutations in the Acetylcholinesterase (AChE) gene using the technique restriction fragment lenght polymorphism in patients with chronic chagas disease
Grantee:André Cardoso Campello
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/14514-4 - Characterization of the chitinase from South American endemic Leishmania species: use in diagnosis in humans, dogs and sandflies.
Grantee:Marcia Aparecida Speranca
Support Opportunities: Regular Research Grants