Tissue Accumulation and the Effects of Long-Term Dietary Copper Contamination on Osmoregulation in the Mudflat Fiddler Crab Minuca rapax (Crustacea, Ocypodidae)

Author(s)

Capparelli, V, M. ; McNamara, J. C. ^{[1, 2]} ; Grosell, M. G. ^[3]

Affiliation

^[1] Univ Sao Paulo, Ctr Biol Marinha, BR-11600000 Sao Sebastiao, SP - Brazil

^[2] Capparelli, M., V, Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Miami, Rosenstiel Sch Marine & Atmospher Sci, Marine Biol & Fisheries, 4600 Rickenbacker Causeway, Miami, FL 33149 - USA

• Document type: Journal article
• Source: Bulletin of Environmental Contamination and Toxicology; v. 104, n. 6 MAY 2020.
• Web of Science Citations: 0

Abstract

We examined copper accumulation in the hemolymph, gills and hepatopancreas, and hemolymph osmolality, Na+ and Cl- concentrations, together with gill Na+/K+-ATPase and carbonic anhydrase activities, after dietary copper delivery (0, 100 or 500 Cu mu g g(-1)) for 12 days in a fiddler crab, Minuca rapax. In contaminated crabs, copper concentration decreased in the hemolymph and hepatopancreas, but increased in the gills. Hemolymph osmolality and gill Na+/K+-ATPase activity increased while hemolymph {[}Na+] and {[}Cl-] and gill carbonic anhydrase activity decreased. Excretion likely accounts for the decreased hemolymph and hepatopancreas copper titers. Dietary copper clearly affected osmoregulatory ability and hemolymph Na+ and Cl- regulation in M. rapax. Gill copper accumulation decreased carbonic anhydrase activity, suggesting that dietary copper affects acid-base balance. Elevated gill Na+/K+-ATPase activity appears to compensate for the ion-regulatory disturbance. These effects of dietary copper illustrate likely impacts on semi-terrestrial species that feed on metal-contaminated sediments. (AU)

• FAPESP's process: 11/22537-0 - Macroevolutionary implications of osmotic, thermal and metabolic homeostasis in inter-tidal, neotropical crabs
• Grantee: John Campbell McNamara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/08065-9 - A qualitative and quantitative analysis of metal pollution in the fiddler crab Uca rapax (Ocypodidae, Brachyura) evaluated using physiological, biochemical and genetic biomarkers
• Grantee: Mariana Vellosa Capparelli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/10672-6 - Physiological and biochemical responses to copper exposure in the fiddler crab Uca rapax (Crustacea, Ocypodidae): different pathways of metal uptake
• Grantee: Mariana Vellosa Capparelli
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate