| Full text | |
| Author(s): |
Cantelmo, Rebeca Araujo
[1]
;
dos Santos, Neife Aparecida G.
[2]
;
dos Santos, Antonio Cardozo
[2]
;
Joca, Samia Regiane Lourenco
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto FCFRP, Dept Biomol Sci, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto FCFRP, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Pharmacy and Pharmacology; v. 72, n. 10 JUN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Objectives To investigate S-adenosyl-methyonine (SAM) effects on PC12 cells viability and neuritogenesis treated with MPP+ (1-methyl-4-phenylpyridinium). Methods PC12 cell viability test (MTT assay) in DMEM medium with SAM and/or MPP+; PC12 cell neuritogenesis test in F-12K medium with nerve growth factor (NGF); DNMT activity in PC12 cells (DNMT Activity Assay Kit) with SAM and/or MPP+. Key findings (1) MPP+ decreased cell viability; (2) SAM did not affect cell viabilityper se, but it increased MPP+ neurotoxicity when co-incubated with the neurotoxin, an effect abolished by DNA methyltransferases (DNMT) inhibitors; (3) pretreatment with SAM for 30 min or 24 h before MPP+ addition had no effect on cell viability. Neuritogenesis: Treatment with SAM for 30 min or 24 h (1) increased cell differentiationper se, (2) increased NGF differentiating effects (additive effect) and (3) blocked the neuritogenesis impairment induced by MPP+. SAM with MPP+ increased the DNMT activity, whereas SAM alone or MPP+ alone did not. Conclusions (1) SAM might induce neurotoxic or neuroprotective effects on PC12 cells, depending on the exposure conditions; (2) DNMT inhibitors might attenuate the MPP+ exacerbation toxicity induced by SAM; (3) DNA methylation might be involved in the observed effects of SAM (needs further investigation). (AU) | |
| FAPESP's process: | 15/05530-3 - The effect of DNA methylation inhibitors on 1-methyl-4-phenylpyridinium iodide (MPP +) induced neurotoxicity in cultured neuronal cells model. |
| Grantee: | Rebeca Araujo Cantelmo |
| Support Opportunities: | Scholarships in Brazil - Master |