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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A proteomic study of myeloproliferative neoplasms using reverse-phase protein arrays

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Tognon, Raquel [1, 2] ; Almeida-e-Silva, Danillo C. [3] ; Andraos-Rey, Rita [4] ; Ristov, Mitko [4] ; Ambrosio, Luciana [1] ; de Almeida, Felipe Campos [1] ; de Souza Nunes, Natalia [1] ; Xisto Souto, Elizabeth [5] ; de Lourdes Perobelli, Leila [5] ; Simoes, Belinda Pinto [6] ; Alexander Guthy, Daniel [4] ; Radimerski, Thomas [4] ; Attie de Castro, Fabiola [1]
Total Authors: 13
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil
[2] Univ Fed Juiz de Fora, Dept Farm, Inst Ciencias Vida, Campus Governador Valadares, Governador Valadares - Brazil
[3] Univ Sao Paulo, LabPIB, Dept Comp & Math FFCLRP USP, Ribeirao Preto - Brazil
[4] Novartis Inst BioMed Res, Dis Area Oncol, Basel - Switzerland
[5] Hosp Estadual Transplantes Euryclides Jesus Zerbi, Sao Paulo - Brazil
[6] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, Ribeirao Preto - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Leukemia & Lymphoma; v. 61, n. 13 AUG 2020.
Web of Science Citations: 0

Myeloproliferative neoplasms polycythemia vera (PV), essential thrombocythaemia (ET) and primary myelofibrosis constitute a group of haematological diseases. The comprehensive assessment of signaling pathway activation in blood cells may aid the understanding of MPN pathophysiology. Thus, levels of post-translational protein modifications and total protein expression were determined in MPN patients and control leukocytes by using reverse-phase protein arrays (RPPA). Compared to control samples, p-SRC, p-CTNNB1, c-MYC, MCL-1, p-MDM2, BAX and CCNB1 showed higher expression in PV samples than controls. P-JAK2/JAK2 and pro-apoptotic BIM showed differential expression betweenJAK2V617F-positive and -negative ET patients. Apoptosis, cancer and PI3K/AKT pathways proteins showed differential expression among the studied groups. For most of the proteins analyzed using Western-Blot and RPPA, RPPA showed higher sensitivity to detect subtle differences. Taken together, our data indicate deregulated protein expression in MPN patients compared to controls. Thus, RPPA may be a useful method for broad proteome analysis in MPN patients ` leukocytes. (AU)

FAPESP's process: 11/51616-6 - Molecular and epigenetic changes of apoptosis, cell cycle and JAK/STAT signaling pathway in JAK2 V617F-positive and negative chronic myeloproliferative neoplasms
Grantee:Raquel Tognon Ribeiro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/20135-2 - Molecular and epigenetic analysis in JAK2 V617F-positive and -negative chronic myeloproliferative neoplasms
Grantee:Fabíola Attié de Castro
Support Opportunities: Regular Research Grants