A proteomic study of myeloproliferative neoplasms using reverse-phase protein arrays

Author(s):Show less -

Tognon, Raquel ^{[1, 2]} ; Almeida-e-Silva, Danillo C. ^[3] ; Andraos-Rey, Rita ^[4] ; Ristov, Mitko ^[4] ; Ambrosio, Luciana ^[1] ; de Almeida, Felipe Campos ^[1] ; de Souza Nunes, Natalia ^[1] ; Xisto Souto, Elizabeth ^[5] ; de Lourdes Perobelli, Leila ^[5] ; Simoes, Belinda Pinto ^[6] ; Alexander Guthy, Daniel ^[4] ; Radimerski, Thomas ^[4] ; Attie de Castro, Fabiola ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Fed Juiz de Fora, Dept Farm, Inst Ciencias Vida, Campus Governador Valadares, Governador Valadares - Brazil

^[3] Univ Sao Paulo, LabPIB, Dept Comp & Math FFCLRP USP, Ribeirao Preto - Brazil

^[4] Novartis Inst BioMed Res, Dis Area Oncol, Basel - Switzerland

^[5] Hosp Estadual Transplantes Euryclides Jesus Zerbi, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, Ribeirao Preto - Brazil

• Document type: Journal article
• Source: Leukemia & Lymphoma; v. 61, n. 13 AUG 2020.
• Web of Science Citations: 0

Abstract

Myeloproliferative neoplasms polycythemia vera (PV), essential thrombocythaemia (ET) and primary myelofibrosis constitute a group of haematological diseases. The comprehensive assessment of signaling pathway activation in blood cells may aid the understanding of MPN pathophysiology. Thus, levels of post-translational protein modifications and total protein expression were determined in MPN patients and control leukocytes by using reverse-phase protein arrays (RPPA). Compared to control samples, p-SRC, p-CTNNB1, c-MYC, MCL-1, p-MDM2, BAX and CCNB1 showed higher expression in PV samples than controls. P-JAK2/JAK2 and pro-apoptotic BIM showed differential expression betweenJAK2V617F-positive and -negative ET patients. Apoptosis, cancer and PI3K/AKT pathways proteins showed differential expression among the studied groups. For most of the proteins analyzed using Western-Blot and RPPA, RPPA showed higher sensitivity to detect subtle differences. Taken together, our data indicate deregulated protein expression in MPN patients compared to controls. Thus, RPPA may be a useful method for broad proteome analysis in MPN patients ` leukocytes. (AU)

• FAPESP's process: 11/51616-6 - Molecular and epigenetic changes of apoptosis, cell cycle and JAK/STAT signaling pathway in JAK2 V617F-positive and negative chronic myeloproliferative neoplasms
• Grantee: Raquel Tognon Ribeiro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/20135-2 - Molecular and epigenetic analysis in JAK2 V617F-positive and -negative chronic myeloproliferative neoplasms
• Grantee: Fabíola Attié de Castro
• Support Opportunities: Regular Research Grants