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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pioneering Study onRhopalurus crassicaudaScorpion Venom: Isolation and Characterization of the Major Toxin and Hyaluronidase

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Author(s):
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Abreu, Caio B. [1] ; Bordon, Karla C. F. [2] ; Cerni, Felipe A. [2] ; Oliveira, Isadora S. [2] ; Balenzuela, Carla [1] ; Alexandre-Silva, Gabriel M. [1] ; Zoccal, Karina F. [3] ; Reis, Mouzarllem B. [4, 3] ; Wiezel, Gisele A. [2] ; Peigneur, Steve [5] ; Pinheiro-Junior, Ernesto L. [5] ; Tytgat, Jan [5] ; Cunha, Tiago M. [6] ; Quinton, Loic [7] ; Faccioli, Lucia H. [4] ; Arantes, Eliane C. [2] ; Zottich, Umberto [1] ; Pucca, Manuela B. [1]
Total Authors: 18
Affiliation:
[1] Univ Fed Roraima, Med Sch, Boa Vista, Parana - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept BioMol Sci, Sao Paulo - Brazil
[3] Barao Maua Univ Ctr, Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo - Brazil
[5] Katholieke Univ Leuven, Toxicol & Pharmacol, Leuven - Belgium
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Sao Paulo - Brazil
[7] Univ Liege, MolSys Res Unit, Mass Spectrometry Lab, Liege - Belgium
Total Affiliations: 7
Document type: Journal article
Source: FRONTIERS IN IMMUNOLOGY; v. 11, AUG 20 2020.
Web of Science Citations: 0
Abstract

Scorpionism is responsible for most accidents involving venomous animals in Brazil, which leads to severe symptoms that can evolve to death. Scorpion venoms consist of complexes cocktails, including peptides, proteins, and non-protein compounds, making separation and purification procedures extremely difficult and time-consuming. Scorpion toxins target different biological systems and can be used in basic science, for clinical, and biotechnological applications. This study is the first to explore the venom content of the unexplored scorpion speciesRhopalurus crassicauda, which inhabits exclusively the northernmost state of Brazil, named Roraima, and southern region of Guyana. Here, we pioneer the fractionation of theR. crassicaudavenom and isolated and characterized a novel scorpion beta-neurotoxin, designated Rc1, and a monomeric hyaluronidase.R. crassicaudavenom and Rc1 (6,882 Da) demonstrated pro-inflammatory activitiesin vitroand a nociceptive responsein vivo. Moreover, Rc1 toxin showed specificity for activating Na(v)1.4, Na(v)1.6, and BgNa(v)1 voltage-gated ion channels. This study also represents a new perspective for the treatment of envenomings in Roraima, since the Brazilian scorpion and arachnid antivenoms were not able to recognizeR. crassicaudavenom and its fractions (with exception of hyaluronidase). Our work provides useful insights for the first understanding of the painful sting and pro-inflammatory effects associated withR. crassicaudaenvenomings. (AU)

FAPESP's process: 17/00586-6 - Characterization of a phospholipase A2 inhibitor from the Crotalus durissus terrificus venom gland: a possible adjuvant in the antivenom therapy
Grantee:Gisele Adriano Wiezel
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/04761-4 - Functional and structural characterization and immune response evaluation of a recombinant serine protease from Crotalus durissus collilineatus modified by PEGylation
Grantee:Ernesto Lopes Pinheiro Junior
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/04724-4 - Improving of human fragment antibodies (scFVs) specific for animals' venoms
Grantee:Eliane Candiani Arantes Braga
Support type: Regular Research Grants
FAPESP's process: 17/03580-9 - Biochemical, structural and functional evaluation of a phosphodiesterase from Crotalus durissus collilineatus venom
Grantee:Isadora Sousa de Oliveira
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/10173-6 - Production, modification and characterization of animal toxins with potential biotechnological application
Grantee:Eliane Candiani Arantes Braga
Support type: Regular Research Grants
FAPESP's process: 17/14035-1 - Improving of human fragment antibodies (scFvs) specific for animals' venoms
Grantee:Felipe Augusto Cerni
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/21233-7 - Mass spectrometry analysis of a new phosphodiesterase from Crotalus durissus collilineatus venom
Grantee:Isadora Sousa de Oliveira
Support type: Scholarships abroad - Research Internship - Doctorate