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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation of intact Leishmania amazonensis large parasitophorous vacuoles from infected macrophages by density gradient fractionation

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Author(s):
Real, Fernando
Total Authors: 1
Document type: Journal article
Source: Experimental Parasitology; v. 218, NOV 2020.
Web of Science Citations: 0
Abstract

As the causative agent of hard-to-treat diffuse cutaneous leishmaniasis, Leishmania (L.) amazonensis persists in the host organism sheltered within large Parasitophorous Vacuoles (PVs) formed mainly in macrophages. In the present study, I present a simple and efficient method for L. amazonensis PV isolation. Isolated PVs are intact as demonstrated by the conservation of lysosomal probes loaded into PVs before the procedure. The method is useful for studies aiming at a complete and accurate molecular profile of these structures, to better understand the biogenesis of this pathogen-containing vacuole and its implication in parasite persistence and in leishmaniasis pathogenesis. (AU)

FAPESP's process: 14/25296-2 - Identification of the minimum requirements for the survival of Leishmania in host cells: RNA-seq of enucleated L929 fibroblasts infected with Leishmania amazonensis
Grantee:Renato Arruda Mortara
Support Opportunities: Regular Research Grants